Regulatory
MioC

Part:BBa_K101017:Design

Designed by: Karl Petersen   Group: iGEM08_Minnesota   (2008-10-27)


MioC Promoter (DNAa-Repressed Promoter)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal SpeI site found at 314
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 314
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal SpeI site found at 314
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 314
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Site directed mutagenesis was used to create a Spe1 restriction site.


Source

The source of this part is from the genomic dna of E.coli (JM109).

References